INVESTIGADORES
MORILLA Maria Jose
artículos
Título:
Avoiding failed reconstitution of ultradeformable liposomes upon dehydration
Autor/es:
J. MONTANARI, D.I. RONCAGLIA, L.A. LADO, M.J. MORILLA, E.L. ROMERO
Revista:
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam; Año: 2009 vol. 372 p. 184 - 190
ISSN:
0378-5173
Resumen:
Although freeze-drying is an ordinarily used technique to dehydrate conventional liposomes, we have
found that ultradeformable liposomes (UDLs) suffered irreversible aggregation when rehydrated upon
freeze-drying (99.4% water elimination), even in high sugar content (4/1 sucrose/lipid mass ratio). When
dehydrated by speed vac and vacuum drying, two alternative techniques that rendered less pronounced
dehydration (94.27 and 96.2% water elimination, respectively) and avoid ice formation, however, UDL
could only be successfully rehydrated when vacuum dried in 4/1 sucrose/lipid mass ratios. Conventional
liposomes, on the other hand, were successfully reconstituted upon dehydrated by the three methods
in lower sugar content (2/1 sucrose/lipid mass ratio). These results indicated that the 27% mole sodium
cholate within the UDL lipid matrix was responsible for a greater and differential mechanical sensitivity
of the bilayers to the different dehydration stress, as compared to conventional liposomes.