Cell migration in BeWo cells and the role of ENaC channels.

DEL MÓNACO SM.; MARINO GI.; ASSEF YA.; DAMIANO AE.; KOTSIAS BA.

JOURNAL OF MEMBRANE BIOLOGY

Springer

Año: 2009 vol. 232 p. 1 - 13

0022-2631

Cell migration/proliferation processes associated with wound healing were measured in BeWo cells at 6 h, when mitosis is still scarce. Cells were cultured in medium with 1% fetal bovine serum to minimize proliferation. BeWo cell migration covered 20.6 ± 7.0%, 38.0 ± 5.4%, 16.6 ± 4.8% and 13.7 ± 3.6% of the wound when cultivated under control, aldosterone (100 nM, 12 h), aldosterone plus amiloride (10 lM) and amiloride treatments, respectively. When BeWo cells were treated with aldosterone, there was an increase in wound healing (P