Trypanosoma cruzi virulence factors for the diagnosis of Chagas disease

CHAIN CECILIA YAMIL; PIRES SOUTO DENIO EMMANUEL; MARIA L SBARAGLINI; LABRIOLA C; DAZA MILLONE ANTONIETA; RAMIREZ EDUARDO ALEJANDRO; CISNEROS JOSE SEBASTIAN; LOPEZ-ALBIZU CONSTANZA; SCOLLO JARENINA; KUBOTA LAURO ; RUIZ ANDRES MARIANO; VELA MARIA ELENA

Infectious Disease

ACS Publications

Lugar: Washintong; Año: 2019

2373-8227

Trans-sialidase and cruzipain are important virulence factors from Trypanosoma cruzi, the etiological agent of Chagas´ disease, that have highly antigenic domains in their structure and were reported as potential tools for diagnosis of the illness. The aim of the present study is to assess the possibility of using cruzipain and the catalytic domain of trans- sialidase in a Surface Plasmon Resonance- based immunosensor for the diagnosis of chronic Chagas´ disease. Immunoassays carried out with canine sera verified that cruzipain, allows the detection of anti- Trypanosoma. cruzi antibodies whereas recombinant trans- sialidase did not yield specific detections, due to the high dilutions of serum used in the immunoassays that hinders the possibility to sense the specific low titer- antibodies. The developed cruzipain- based biosensor, whose price per assay is comparable to commercial ELISA, was successfully applied for the rapid quantification of specific antibodies against Trypanosoma cruzi in fresh human sera showing an excellent agreement with ELISA.