INVESTIGADORES
GARRIZ Andres
artículos
Título:
Defense responses in two ecotypes of Lotus japonicus against non-pathogenic Pseudomonas syringae
Autor/es:
BORDENAVE C; ESCARAY F; MENENDEZ AB; SERNA E; CARRASCO P; RUIZ OA ; GARRIZ A
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2013 p. 1 - 16
ISSN:
1932-6203
Resumen:
Lotus japonicus is a model legume broadly used to study many important processes as
nitrogen fixing nodule formation and adaptation to salt stress. However, no studies on
the defense responses occurring in this species against invading microorganisms have
been carried out at the present. Understanding how this model plant protects itself
against pathogens will certainly help to develop more tolerant cultivars in economically
important Lotus species as well as in other legumes. In order to uncover the most
important defense mechanisms activated upon bacterial attack, we explored in this
work the main responses occurring in the phenotypically contrasting ecotypes MG-20
and Gifu B-129 of L. japonicus after inoculation with Pseudomonas syringae DC3000
pv. tomato. Our analysis demonstrated that this bacterial strain is unable to cause
disease in these accessions, even though the defense mechanisms triggered in these
ecotypes might differ. Thus, disease tolerance in MG-20 was characterized by bacterial
multiplication, chlorosis and desiccation at the infiltrated tissues. In turn, Gifu B-129
plants did not show any symptom at all and were completely successful in restricting
bacterial growth. We performed a microarray based analysis of these responses and
determined the regulation of several genes that could play important roles in plant
defense. Interestingly, we were also able to identify a set of defense genes with a
relative high expression in Gifu B-129 plants under non-stress conditions, what could
explain its higher tolerance. The participation of these genes in plant defense is
discussed. Our results position the L. japonicus-P. syringae interaction as a interesting
model to study defense mechanisms in legume species.