Immunocompetence of macrophages in rats exposed to Candida albicans infection and stress.

RODRIGUEZ- GALÁN MC; SOTOMAYOR CE; COSTAMAGNA ME; CABANILLAS AM; SALIDO- RENTARÍA B; MASINI DE REPISO; CORREA SG

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

AMER PHYSIOLOGICAL SOC

Año: 2003 vol. 284 p. 111 - 118

0363-6143

The integration of innate and adaptive immune responses is required for efficient control of Candida albicans. The present work aimed to assess, at the local site of the infection, the immunocompetence of macrophages in rats infected intraperitoneally with C. albicans and exposed simultaneously to stress during 3 days (CaS group). We studied the 1) ability to remove and kill C. albicans, 2) tumor necrosis factor-
Candida albicans. The present work aimed to assess, at the local site of the infection, the immunocompetence of macrophages in rats infected intraperitoneally with C. albicans and exposed simultaneously to stress during 3 days (CaS group). We studied the 1) ability to remove and kill C. albicans, 2) tumor necrosis factor-
C. albicans and exposed simultaneously to stress during 3 days (CaS group). We studied the 1) ability to remove and kill C. albicans, 2) tumor necrosis factor-
1) ability to remove and kill C. albicans, 2) tumor necrosis factor-
C. albicans, 2) tumor necrosis factor-
(TNF-
) release, 3) balance of the inducible enzymes NO synthase (iNOS) and arginase, and 4) expression of interleukin (IL)-1 and IL-1 receptor antagonist (ra) mRNA. Compared with only infected animals (Ca group), the number of colony-forming units was significantly higher in CaS rats (P  0.01), and the macrophage candidicidal activity was
) release, 3) balance of the inducible enzymes NO synthase (iNOS) and arginase, and 4) expression of interleukin (IL)-1 and IL-1 receptor antagonist (ra) mRNA. Compared with only infected animals (Ca group), the number of colony-forming units was significantly higher in CaS rats (P  0.01), and the macrophage candidicidal activity was4) expression of interleukin (IL)-1 and IL-1 receptor antagonist (ra) mRNA. Compared with only infected animals (Ca group), the number of colony-forming units was significantly higher in CaS rats (P  0.01), and the macrophage candidicidal activity was and IL-1 receptor antagonist (ra) mRNA. Compared with only infected animals (Ca group), the number of colony-forming units was significantly higher in CaS rats (P  0.01), and the macrophage candidicidal activity wasP  0.01), and the macrophage candidicidal activity was 2.5-fold lower (P  0.01). Release of TNF-
was diminished in both unstimulated and heat-killed C. albicans restimulated macrophages of the CaS group (Ca vs. CaS, P  0.03 and P  0.05, respectively). In Ca- and CaS-group rats, the rates for both the arginase activity and the NO synthesis were significantly enhanced. However, the stress exposure downregulated the activity of both enzymes (CaS vs. Ca, P 2.5-fold lower (P  0.01). Release of TNF-
was diminished in both unstimulated and heat-killed C. albicans restimulated macrophages of the CaS group (Ca vs. CaS, P  0.03 and P  0.05, respectively). In Ca- and CaS-group rats, the rates for both the arginase activity and the NO synthesis were significantly enhanced. However, the stress exposure downregulated the activity of both enzymes (CaS vs. Ca, P C. albicans restimulated macrophages of the CaS group (Ca vs. CaS, P  0.03 and P  0.05, respectively). In Ca- and CaS-group rats, the rates for both the arginase activity and the NO synthesis were significantly enhanced. However, the stress exposure downregulated the activity of both enzymes (CaS vs. Ca, P P  0.03 and P  0.05, respectively). In Ca- and CaS-group rats, the rates for both the arginase activity and the NO synthesis were significantly enhanced. However, the stress exposure downregulated the activity of both enzymes (CaS vs. Ca, P P  0.05, respectively). In Ca- and CaS-group rats, the rates for both the arginase activity and the NO synthesis were significantly enhanced. However, the stress exposure downregulated the activity of both enzymes (CaS vs. Ca, P P  0.05). After in vitro restimulation, the IL-1ra/IL-1 ratio was significantly diminished in CaS-group rats (P  0.05). Our results indicate that a correlation exists between early impairment of macrophage function and stress exposure. ratio was significantly diminished in CaS-group rats (P  0.05). Our results indicate that a correlation exists between early impairment of macrophage function and stress exposure.P  0.05). Our results indicate that a correlation exists between early impairment of macrophage function and stress exposure.