CERZOS   05458
CENTRO DE RECURSOS NATURALES RENOVABLES DE LA ZONA SEMIARIDA
Unidad Ejecutora - UE
artículos
Título:
Variation in cytosine methylation patterns during ploidy level conversions in Eragrostis curvula
Autor/es:
OCHOGAVIA A; CERVIGNI, G.; SELVA, J.P.; ECHENIQUE V; PESSINO, S.
Revista:
PLANT MOLECULAR BIOLOGY
Editorial:
Springer
Referencias:
Lugar: Netherlands; Año: 2009 vol. 70 p. 17 - 29
ISSN:
0167-4412
Resumen:
Abstract In many species polyploidization involves
rearrangements of the progenitor genomes, at both genetic
and epigenetic levels. We analyzed the cytosine methylation
status in a tetraploid-diploid-tetraploid series of Eragrostis
curvula with a common genetic background by using the
MSAP (Methylation-sensitive Amplified Polymorphism)
technique. Considerable levels of polymorphisms were
detected during ploidy conversions. The total level of
methylation observed was lower in the diploid genotype
compared to the tetraploid ones. A significant proportion of
the epigenetic modifications occurring during the tetraploid
diploid conversion reverted during the diploid
tetraploid one. Genetic and expression data from previous
work were used to analyze correlation with methylation
variation. All genetic, epigenetic and gene expression variation
data correlated significantly when compared by pairs
in simple Mantel tests. Dendrograms reflecting genetic,
epigenetic and expression distances as well as principal
coordinate analysis suggested that plants of identical ploidy
levels present similar sets of data. Twelve (12) different
genomic fragments displaying different methylation
behavior during the ploidy conversions were isolated,
sequenced and characterized
MSAP (Methylation-sensitive Amplified Polymorphism)
technique. Considerable levels of polymorphisms were
detected during ploidy conversions. The total level of
methylation observed was lower in the diploid genotype
compared to the tetraploid ones. A significant proportion of
the epigenetic modifications occurring during the tetraploid
diploid conversion reverted during the diploid
tetraploid one. Genetic and expression data from previous
work were used to analyze correlation with methylation
variation. All genetic, epigenetic and gene expression variation
data correlated significantly when compared by pairs
in simple Mantel tests. Dendrograms reflecting genetic,
epigenetic and expression distances as well as principal
coordinate analysis suggested that plants of identical ploidy
levels present similar sets of data. Twelve (12) different
genomic fragments displaying different methylation
behavior during the ploidy conversions were isolated,
sequenced and characterized
curvula with a common genetic background by using the
MSAP (Methylation-sensitive Amplified Polymorphism)
technique. Considerable levels of polymorphisms were
detected during ploidy conversions. The total level of
methylation observed was lower in the diploid genotype
compared to the tetraploid ones. A significant proportion of
the epigenetic modifications occurring during the tetraploid
diploid conversion reverted during the diploid
tetraploid one. Genetic and expression data from previous
work were used to analyze correlation with methylation
variation. All genetic, epigenetic and gene expression variation
data correlated significantly when compared by pairs
in simple Mantel tests. Dendrograms reflecting genetic,
epigenetic and expression distances as well as principal
coordinate analysis suggested that plants of identical ploidy
levels present similar sets of data. Twelve (12) different
genomic fragments displaying different methylation
behavior during the ploidy conversions were isolated,
sequenced and characterized
MSAP (Methylation-sensitive Amplified Polymorphism)
technique. Considerable levels of polymorphisms were
detected during ploidy conversions. The total level of
methylation observed was lower in the diploid genotype
compared to the tetraploid ones. A significant proportion of
the epigenetic modifications occurring during the tetraploid
diploid conversion reverted during the diploid
tetraploid one. Genetic and expression data from previous
work were used to analyze correlation with methylation
variation. All genetic, epigenetic and gene expression variation
data correlated significantly when compared by pairs
in simple Mantel tests. Dendrograms reflecting genetic,
epigenetic and expression distances as well as principal
coordinate analysis suggested that plants of identical ploidy
levels present similar sets of data. Twelve (12) different
genomic fragments displaying different methylation
behavior during the ploidy conversions were isolated,
sequenced and characterized
rearrangements of the progenitor genomes, at both genetic
and epigenetic levels. We analyzed the cytosine methylation
status in a tetraploid-diploid-tetraploid series of Eragrostis
curvula with a common genetic background by using the
MSAP (Methylation-sensitive Amplified Polymorphism)
technique. Considerable levels of polymorphisms were
detected during ploidy conversions. The total level of
methylation observed was lower in the diploid genotype
compared to the tetraploid ones. A significant proportion of
the epigenetic modifications occurring during the tetraploid
diploid conversion reverted during the diploid
tetraploid one. Genetic and expression data from previous
work were used to analyze correlation with methylation
variation. All genetic, epigenetic and gene expression variation
data correlated significantly when compared by pairs
in simple Mantel tests. Dendrograms reflecting genetic,
epigenetic and expression distances as well as principal
coordinate analysis suggested that plants of identical ploidy
levels present similar sets of data. Twelve (12) different
genomic fragments displaying different methylation
behavior during the ploidy conversions were isolated,
sequenced and characterized
MSAP (Methylation-sensitive Amplified Polymorphism)
technique. Considerable levels of polymorphisms were
detected during ploidy conversions. The total level of
methylation observed was lower in the diploid genotype
compared to the tetraploid ones. A significant proportion of
the epigenetic modifications occurring during the tetraploid
diploid conversion reverted during the diploid
tetraploid one. Genetic and expression data from previous
work were used to analyze correlation with methylation
variation. All genetic, epigenetic and gene expression variation
data correlated significantly when compared by pairs
in simple Mantel tests. Dendrograms reflecting genetic,
epigenetic and expression distances as well as principal
coordinate analysis suggested that plants of identical ploidy
levels present similar sets of data. Twelve (12) different
genomic fragments displaying different methylation
behavior during the ploidy conversions were isolated,
sequenced and characterized
curvula with a common genetic background by using the
MSAP (Methylation-sensitive Amplified Polymorphism)
technique. Considerable levels of polymorphisms were
detected during ploidy conversions. The total level of
methylation observed was lower in the diploid genotype
compared to the tetraploid ones. A significant proportion of
the epigenetic modifications occurring during the tetraploid
diploid conversion reverted during the diploid
tetraploid one. Genetic and expression data from previous
work were used to analyze correlation with methylation
variation. All genetic, epigenetic and gene expression variation
data correlated significantly when compared by pairs
in simple Mantel tests. Dendrograms reflecting genetic,
epigenetic and expression distances as well as principal
coordinate analysis suggested that plants of identical ploidy
levels present similar sets of data. Twelve (12) different
genomic fragments displaying different methylation
behavior during the ploidy conversions were isolated,
sequenced and characterized
MSAP (Methylation-sensitive Amplified Polymorphism)
technique. Considerable levels of polymorphisms were
detected during ploidy conversions. The total level of
methylation observed was lower in the diploid genotype
compared to the tetraploid ones. A significant proportion of
the epigenetic modifications occurring during the tetraploid
diploid conversion reverted during the diploid
tetraploid one. Genetic and expression data from previous
work were used to analyze correlation with methylation
variation. All genetic, epigenetic and gene expression variation
data correlated significantly when compared by pairs
in simple Mantel tests. Dendrograms reflecting genetic,
epigenetic and expression distances as well as principal
coordinate analysis suggested that plants of identical ploidy
levels present similar sets of data. Twelve (12) different
genomic fragments displaying different methylation
behavior during the ploidy conversions were isolated,
sequenced and characterized