CONICET | Buscador de Institutos y Recursos Humanos

Shiga toxin-producing Escherichia coli (STEC) are emerging pathogens associated with severe and fatal disease in children as Hemolytic Uremic Syndrome (HUS).These bacteria are shedding with feces of cattle contaminating the environment and would enter the food chain if the slaughter process is not done correctly.The prevention measures and control strategies are the key tools to reduce the transmission of STEC. Oral bismuth hydroxide gel has been widely used as antidiarrheal. In this study, the effects of Bismuth hydroxide gel on culture of STEC O157:H7, 026:H11 and O103:H2 was assayed. To evaluate the effects on the viability of STEC O157:H7, O91:H21 and O26:H11 on glass surfaces, two types of novel Bismuth hydroxide presentations, emulsion spray and aerosol were assayed. STEC strains were cultured in LB broth and Bismuth hydroxide gel (Soubeiran Chobet, S.R.L., and City of Buenos Aires, Argentina) was added to eachplate. At different times, an aliquote of each culture were plated onto MacConkey agar for colony counts. To evaluate the effects on the viability of STEC O157 and non-O157 strains on glass surfaces, Bismuth hydroxide gel emulsion spray and aerosol was independently sprayed on sterile glass plates previously scattered with STEC O157:H7, O91:H21 and O26:H11. The effects were determined at different times by swabbing on MacConkey agar plates and counting CFU. In both assays, STEC strains without the addition of bismuth hydroxide gel were used as controls.All the STEC strains were affected in their growth after the application of Bismuth hydroxide gel in LB broth. Bismuth spray and aerosol were effective for SETC viability on surfaces although the spray showed more efficiency than the aerosol. Since that contaminated surfaces with STEC represent a risk in the food industry, Bismuth Hydroxide gel in these novel presentations is promising as decontaminant on inert surfaces.

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