CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
artículos
Título:
Endocannabinoid system and nitric oxide are involved in the deleterious effects of lipopolysaccharide on murine decidua.
Autor/es:
VERCELLI CA; AISEMBERG J; BILLI S; WOLFSON ML; FRANCHI AM
Revista:
PLACENTA
Editorial:
W.B. Saunders
Referencias:
Lugar: England; Año: 2009 vol. 30 p. 579 - 584
ISSN:
0143-4004
Resumen:
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Endocannabinoids are an important family of
lipid-signaling molecules that are widely distributed in
mammalian tissues and anandamide (AEA) was the first
member identified. The uterus contains the
highest concentrations of AEA yet discovered in mammalian
tissues and this suggests that it might play a role in reproduction. Previous
results from our laboratory have shown that AEA modulated NO synthesis in rat
placenta. The production of small amounts of nitric oxide regulates various
physiological reproductive processes such as implantation, decidualization and
myometrial relaxation. But in an inflammatory setting such as sepsis, NO is
produced in big amounts and has toxic effects as it is a free radical. The
results presented in this study indicate that LPS-induced NO synthesis and
tissue damage were mediated by AEA. Decidual LPS-induced NO production was
abrogated either by co-incubation with CB1 (AM251) or CB2 (SR144528)
antagonists which suggests that both receptors could be mediating this effect. On
the other hand, LPS-induced tissue damage and this deleterious effect was
partially abrogated by incubating tissue explants with LPS plus CB1 receptor
antagonist. Our findings suggest that AEA, probably by increasing NO synthesis,
participates in the deleterious effect of LPS in implantation sites. These
effects could be involved in pathological reproductive events such as septic
abortion.