INVESTIGADORES
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artículos
Título:
A Novel and Rapid Method for Determination of Natamycin in Wines based on Ultra High Performance Liquid Chromatography coupled to Tandem Mass Spectrometry. Validation According to the 2002/657/EC European Decision
Autor/es:
L. MARIÑO REPIZO, L.D. MARTINEZ, R.A. OLSINA, S. CERUTTI, J. RABA
Revista:
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Editorial:
SPRINGER HEIDELBERG
Referencias:
Lugar: HEIDELBERG; Año: 2012 vol. 402 p. 965 - 973
ISSN:
1618-2642
Resumen:
A novel, simple, and rapid reversed-phase liquid chromatographytandem
mass spectrometric methodology was developed for the
analysis of natamycin in wine samples. Natamycin was
protonated to form singly charged ions in an electrospray positive ion
mode. Data acquisition under MS/MS was achieved by applying
multiple reaction monitoring (MRM) of three fragment ion transitions
(666.3 → 648.2, 666.3 → 503.3, and 666.3 → 485.2) to provide
a high degree of sensitivity and specificity. Chromatographic
separation was performed on a rapid resolution column using a
mobile phase consisting of an acetonitrile/water mixture with
a total run time of 5.0 min. After only filtration as
pretreatment, the sample was injected into the chromatographic system.
The proposed method was validated in terms of selectivity,
trueness, precision, decision limit (CCα), and detection capability (CCβ)
according to 2002/657/EC Commission decision. The values for trueness,
reported as bias (%), agreed with those established
by the aforementioned document. Repeatability (intraday
variability) values were 12.37% at a concentration of 1.0 μg L−1 and 8.994.19% at concentrations between 2.5 and 10 μg L−1. The overall within-laboratory (interday variability) reproducibility was 15.47% at a concentration of 1.0 μg L−1,
which was significantly lower than the indicative value reported in the
EU decision. The results indicated that the proposed
approach is a sensitive, fast, reproducible, and robust
methodology suitable for the analysis of natamycin levels in wine
samples.