Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: Neuroepigenetic implications.

BISAGNO, VERONICA; URBANO, FRANCISCO J.; GARCIA-RILL, EDGAR

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

AMER PHYSIOLOGICAL SOC

Año: 2020 vol. 318 p. 282 - 288

0363-6143

The pedunculopontine nucleus (PPN) is part of the reticular activating system (RAS) in charge of arousal and REM sleep. The presence of high frequency membrane oscillations in the gamma band range in the PPN has been extensively demonstrated both in vivo and in vitro. Our group previously described HDAC inhibition in vitro induced protein changes in F-actin cytoskeleton and intracellular [Ca2+] regulation proteins in the PPN. Here, we present evidence that supports the presence of a fine balance between histone deacetylation (HDAC) function and CaMKII-F-actin interactions in the PPN. We modified F-actin polymerization in vitro by using jasplakinolide (JAS, 1 microM, a promoter of F-actin stabilization), or latrunculin-B (LAT-B, 1 microM, an inhibitor of actin polymerization). Our results showed that shifting the balance in either direction significantly reduced PPN gamma oscillation as well as voltage-dependent calcium currents.