INVESTIGADORES
REPETTO Marisa Gabriela
artículos
Título:
Neutrophyl function nitric oxide and blood oxidative stress in Parkinson´s Disease.
Autor/es:
CARRERAS, MARÍA CECILIA; PARGAMENT, GRISELDA; RIOBÓ, C; REIDES, CLAUDIA; REPETTO, MARISA; FERNANDEZ PARDAL, M.; LLESUY, SUSANA; PODEROSO, JUAN JOSÉ
Revista:
Focus in Parkinson disease
Editorial:
Bugamor Bv
Referencias:
Año: 1997 vol. 9 p. 12 - 14
ISSN:
0924-2015
Resumen:
Some biochemical facts, such as a markedly increased NO release of PMNs and low erythrocyte catalase activity, are stereotypically present from the beginning of Parkinson disease and suggest a more generalized, but still unproven, metabolic defect in Parkinson, that may serve as an accessible peripheral marker for these patients. We studied nitrogen radical nitric oxide (.NO) release and reactive oxygen species (ROS) production by isolated neutrophils after phorbol myristate acetate (PMA) stimulation in 12 newly diagnosed and nine treated Parkinson´s disease (PD) patients and 10 age-matched healthy controls. Neutrophils of both groups of PD patients had an elevated PMA-activated release of .NO [61 and 57%, respectively, higher than that of controls (p < 0.05)]. In contrast, H2O2 release was only significantly increased by 56% in chronically treated patients. In agreement, the maximum rate of luminol-dependent chemiluminescence, which partly represents H2O2/NO interactions, was increased only in the treated group. When other blood markers of oxidative stress were compared, only erythrocyte catalase activity was decreased in both PD patient series by 33 and 39%, respectively (p < 0.05), whereas plasma antioxidant capacity and erythrocyte superoxide dismutase activity levels were decreased only in treated PD patients. This study suggests that neutrophils express a primary alteration of .NO release in PD patients, whereas H2O2 and oxidative-stress parameters are more probably related to the evolution of PD or to effects of treatment with L-dopa.