4-nerolydilcatechol from Pothomorphe spp. inhibits in vitro generation of peroxyl radicals and Fe (II)-dependent DNA damage

DESMARCHELIER, CRISTIAN; REPETTO, MARISA; COUSSIO, JORGE; RIBEIRO LATORRE, L; KATO, M; BARROS, SILVIA; CICCIA, GRACIELA

PLANTA MEDICA

GEORG THIEME VERLAG KG

Lugar: Stuttgart; Año: 1997 vol. 63 p. 561 - 563

0032-0943

The total reactive antioxidant potential (TRAP) and total antioxidant reactivity (TAR) of 4-nerolidylcatechol (4-NC) and methanolic extracts of Pothomorphe umbellata and P. peltata were determined by monitoring the intensity of luminol enhanced chemiluminescence by peroxyl radicals derived from thermolysis of 2,2´-azobis(2-amidinopropane). The highest antioxidant potential was measured in the extract of P. umbellata (TRAP = 97.2 uM) while the highest reactivity was observed in the extract of P. peltata (TAR = 5.0 uM), measured as equivalents of Trolox concentration. These results were higher than those obtained for 4-NC (TRAP = 33.6 uM, TAR = 4.9 uM). DNA sugar damage induced by Fe(II) salts was also used to determine the capacity of 4-NC to suppress hydroxyl radical-mediated degradation of DNA. Calculated IC50 values for 4-NC and catechin, used as a standard, were 25 and 17uM, respectively.