INVESTIGADORES
REPETTO Marisa Gabriela
artículos
Título:
Protective effect of menhaden oil in the oxidative damage and renal necrosis due to dietary choline deficiency
Autor/es:
OSSANI, GEORGINA; REPETTO, MARISA; BOVERIS, ALBERTO; MONSERRAT, ALBERTO
Revista:
Food and function
Editorial:
RSC Publishing. Royal Society of Chemistry
Referencias:
Lugar: Washington DC; Año: 2013 vol. 4 p. 448 - 452
ISSN:
2042-6496
Resumen:
Weanling rats fed a choline-deficient (CD) diet develop kidney oxidative damage, tubular and cortical kidney necrosis, renal failure and animal death. The effect of dietary menhaden oil was assayed on the mentioned sequence. Rats were fed ad libitum 4 different diets: (a) a CD diet with corn oil and sunflower hydrogenated oil as source of fatty acids; (b) the same diet suplemented with choline; (c) a CD diet with menhaden oil from Brevoortia tyrannis as source of fatty acids; and (d) the previous diet supplemented with choline. Animals were sacrificed at days 0, 2, 4 and 7. Menhaden oil prevented mortality in CD animals up to day 21 (more than 128 %). The histopathologycal study of the kidneys showed that renal necrosis was only observed at day 7 in CD rats receiving the vegetable oil diet, simultaneously with increased creatinine plasma levels. Homogenate chemiluminescence (BOOH-initiated chemiluminescence) and phospholipid oxidation indicate the development of oxidative stress and damage in CD as well as the protective effect of menhaden oil. Rats fed with the fish oil diet showed that oxidative stress and damage develop later, as co