Oxidative damage is the biochemical mechanism of cellular injury and necrosis in choline deficiency

REPETTO, M.; OSSANI, G.; MONSERRAT, A.; BOVERIS, A

EXPERIMENTAL AND MOLECULAR PATHOLOGY.

Elsevier, Academic Press

Lugar: NETHERLANDS; Año: 2010 vol. 88 p. 143 - 149

0014-4800

Oxidative stress and damage are characterized by decreased tissue antioxidant levels, consumption of tissue tocopherol and increased lipid peroxidation. These processes occur earlier than necrosis in the liver, heart, kidney and brain of weanling rats fed a choline deficient (CD) diet. In tissues, water-soluble antioxidants were analyzed as total reactive antioxidant potential (TRAP), tocopherol content was estimated from homogenate chemiluminescence (homogenate-CL), and lipid peroxidation was determined by thiobarbituric acid reactive substances (TBARS). Histopathology showed hepatic steatosis at days 1-7, tubular and glomerular necrosis in kidney at days 6 and 7, and inflammation and necrosis in heart at days 6 and 7. TRAP levels decreased by 18%, 48%, 56% and 66% at day 7, with t1/2 (times for half maximal change) of 2.0, 1.8, 2.5 and 3.0 days in liver, kidney, heart and brain, respectively. Homogenate-CL increased by 97%, 113%, 18% and 297% at day 7, with TBARS contents increased by 98>%, 157%, 104% and 347% at day 7, with t1/2 of 2.6, 2.8, 3.0 and 5.0 days in the four organs, respectively. Plasma showed a 33% decrease in TRAP and a 5-fold increase in TBARS at day 5. Oxidative stress and damage are processes occurring earlier than necrosis in kidney and heart. In case of liver, steatosis prior to antioxidant consumption and increased lipid peroxidation are observed but without necrosis.