DECAY-ACCELERATING FACTOR 1 DEFICIENCY EXACERBATE Trypanosoma cruzi-INDUCED MURINE CHRONIC MYOSITIS

SOLANA ME, FERRER MF; NOVOA MM; WEN-CHAO S; GOMEZ RM

MUSCLE & NERVE

JOHN WILEY & SONS INC

Lugar: New York; Año: 2012 vol. 46 p. 582 - 587

0148-639X

ABSTRACT: Introduction: Murine infection with Trypanosoma cruzi (Tc) has been used to study the role of T-cells in the pathogenesis of human inflammatory idiopathic myositis. Absence of decay-accelerating factor 1 (Daf1) has been shown to enhance murine T-cell responses and autoimmunity. Methods: To determine whether Daf1 deficiency can exacerbate Tc induced myositis, C57BL/6 DAF +/+ and DAF -/- mice were inoculated with 5x10exp4 trypomastigotes, and their morbidity, parasitemia, parasite burden, histopathology, and T-cell expansion were studied in the acute and chronic stages. Results:DAF mice had lower parasitemia and parasite burden but higher morbidity, muscle histopathology, and increased number of CD44+ (activated/memory phenotype) splenic CD4 T-cells. Conclusions: An enhanced CD8+ T-cell immune-specific response may explain the lower parasitemia and parasite burden levels and the increase in histopathological lesions. We propose that Tc-inoculated DAF-/- mice are a useful model to study T-cell mediated immunity in skeletal muscle tissues.