INPA 24560
UNIDAD EJECUTORA DE INVESTIGACIONES EN PRODUCCION ANIMAL
Unidad Ejecutora - UE
artículos
Título:
Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch
Autor/es:
EDISON CARVALHO; MANUEL DÍAZ; LORENZO MARQUEZ; GABRIEL MORALES; FRANCISCO JAVIER MOYANO LÓPEZ; ADRIÁN HERNÁNDEZ; PATRICIO DANTAGNAN
Revista:
LATIN AMERICAN JOURNAL OF AQUATIC RESEARCH
Editorial:
UNIV CATOLICA DE VALPARAISO
Referencias:
Lugar: Valparaíso; Año: 2019 vol. 47 p. 860 - 864
ISSN:
0718-560X
Resumen:
The activity of fish acid proteases is strongly dependent on the pH, and many basic and appliedworks had focused on researching the pH-profile of digestive proteases using different buffered media.Nevertheless, enzymatic activity is also known to be affected by the ionic strength (IS) of the medium. Thepresent work is aimed at researching the combined effects of pH (2.5, 3.0, 3.5, 3.75 and 4.0) and IS (50, 100 and200 mM) on the protease activity of gastric extracts of juvenile Coho salmon Oncorhynchus kisutch. It isconcluded that the activity of Coho salmon gastric proteases is maximal at pH 2.5-3.0, drops towards pH 4.0irrespective of ionic strength, and they showed a lower activity at IS 50 mM in comparison to IS 100 or 200mM. The present results point out to the convenience of considering the effect of ionic strength when measuringthe activity of fish gastric proteases.
