Detection of Echinococcus granulosus sensu lato infection by using extracts derived from a protoscoleces G1 cell line

MAGLIOCO, ANDREA; GENTILE, JORGE; BARBERY VENTURI, MELISA S.; JENSEN, OSCAR; HERNÁNDEZ, CLAUDIA; GERTISER, MARÍA LAURA; POGGIO, VERÓNICA; CANZIANI, GABRIELA; FUCHS, ALICIA GRACIELA

PARASITE IMMUNOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2019

0141-9838

Cystic echinococcosis (CE) can be diagnosed by means of several serological approaches, but their results vary among laboratories due to the molecular characteristics of the reference antigens used. Thus, this study aimed to address both the relevance of an EGPE cell line previously obtained from Echinococcus granulosus protoscoleces G1 and the complexity of the immune response by using two different in vitro growth stages as separate sources of parasite antigens. The serum reactivity was investigated by western blotting (WB) in 21 CE patients from an endemic area in a matched case-control design and also in seven experimentally infected sheep and five healthy control sheep. EGPE-antigen-human serum sensitivity by WB was higher than that of hydatid fluid (HF) WB, ELISA and DD5 (P