Losing reproduction: effect of high temperature on female biochemical composition and egg quality in a freshwater crustacean with direct development, the red cherry shrimp Neocaridina davidi (Decapoda, Atyidae)

SOFÍA BALIÑA; BRENDA TEMPERONI; LAURA S. LÓPEZ GRECO; CAROLINA TROPEA

BIOLOGICAL BULLETIN

MARINE BIOLOGICAL LABORATORY

Lugar: Woods Hole; Año: 2018 vol. 234 p. 139 - 151

0006-3185

Maternal provisioning is particularly important in invertebrates with abbreviated development because large energy reserves must be provided for the developing embryo. In this context, the objective of the present study was to analyze the effect of temperature on female biochemical composition and reserve allocation to maturing ovaries, which determine egg quality, in an aquatic invertebrate with direct development. A decapod crustacean, the freshwater shrimp Neocaridina davidi, was used as experimental model. Newly hatched juveniles were exposed to 28°C or 33°C. Females matured their ovaries and spawned at 28°C (control ovigerous females), but no ovigerous female was found at 33°C. After a 200-day period, half of the females at 33°C were transferred to 28°C, where they rapidly matured their ovaries and spawned (transferred ovigerous females). Ovigerous females and females that did not spawn at 28°C (control non-ovigerous females) and at 33°C (high temperature non-ovigerous females), were sacrificed to determine their biochemical composition. The number, volume, weight and biochemical composition of the eggs from transferred and control ovigerous females were also analyzed as indicators of their quality. Female biochemical composition was not influenced by temperature, as control and high temperature non-ovigerous females had similar lipid, protein and glycogen contents. However, ovarian maturation and spawning were inhibited at 33ºC, which indicates a negative effect of this temperature on nutrient transfer to the oocytes. This effect was rapidly reversed after females were moved to 28ºC; the eggs from control and transferred ovigerous females were of similar quality, except for a lower protein content in the latter. Present results provide valuable information on reserve allocation to reproduction under thermal stress.