Combinatorial biosynthesis of antimicrobials and other natural products

EDUARDO RODRIGUEZ; ROBERT MCDANIEL

CURRENT OPINION IN MICROBIOLOGY

CURRENT BIOLOGY LTD

Lugar: Londres; Año: 2001 vol. 4 p. 526 - 534

1369-5274

Combinatorial biosynthesis utilizes the enzymes from antibiotic (and other natural product) biosynthetic pathways to create novel chemical structures. The manipulation of modular polyketide synthases (PKSs) has been the major focus of this effort and has led to the production of, for example, several erythromycin analogs. Many new tools for manipulating and studying these multifunctional enzymes have been developed. These include multiple hosts and expression systems, enzymology tools for in vitro study, and ways to engineer pre-PKS and post-PKS pathways. The result is more rationaland faster methods of engineering new compounds for the development of chemotherapeutic agents from natural products. The most significant recent advances in combinatorial biosynthesis are outlined.