Species delimitation in the Andean grasshopper genus Orotettix Ronderos & Carbonell (Orthoptera: Melanoplinae): an integrative approach combining morphological, molecular, and biogeographic data.

POCCO, M.E.; MINUTOLO, C.; DINGHI, P.A.; LANGE, C.E.; CONFALONIERI V.A.; CIGLIANO, M.M.

ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2015 vol. 174 p. 733 - 759

0024-4082

Abstract The reciprocal illumination nature of integrative taxonomy through hypothesis testing, corroboration, and revision is a powerful tool for species delimitation since more than one source has to support the hypothesis of a new species. In this study, we applied an integrative taxonomy approach combining molecular and morphological data sets with distributional patterns to examine the level of differentiation between and within the grasshopper Orotettix species. Orotettix was described based on five valid species distributed in the Andes of Perú. In our study, initially a molecular-based hypothesis was postulated and tested against morphological data and geographic patterns of distribution. Results from molecular and morphological analyses showed agreement among the species delimitation in Orotettix which revealed to be consistent with the geographic distribution. The analyses allowed us to delimit five new species for the genus (O. lunatus n.sp., O. astreptos n.sp., O. colcaensis n.sp., O. paucartambensis n.sp., O. dichrous n.sp.) from the Eastern and Western Cordillera of Perú and the bolivian Altiplano. Besides, we provide critical knowledge on the phylogenetic relationships and distribution of the genus and conduct a revision of Orotettix.