INVESTIGADORES
DE NICOLA Alejandro Federico
artículos
Título:
Neuropathic pain and temporal expression of preprodynorphin, protein kinase C and N-methyl-d aspartate receptor subunits alter spinal cord injury.
Autor/es:
LABOMBARDA F.; CORONEL F.; VILLAR M.; DE NICOLA A.F.; GONZALEZ S.
Revista:
NEUROSCIENCE LETTERS
Editorial:
Elsevier
Referencias:
Lugar: UK; Año: 2008 vol. 447 p. 115 - 119
ISSN:
0304-3940
Resumen:
<!--
/* Font Definitions */
@font-face
{font-family:"MS Mincho";
panose-1:2 2 6 9 4 2 5 8 3 4;
mso-font-alt:"MS 明朝";
mso-font-charset:128;
mso-generic-font-family:modern;
mso-font-pitch:fixed;
mso-font-signature:-1610612033 1757936891 16 0 131231 0;}
@font-face
{font-family:"\@MS Mincho";
panose-1:2 2 6 9 4 2 5 8 3 4;
mso-font-charset:128;
mso-generic-font-family:modern;
mso-font-pitch:fixed;
mso-font-signature:-1610612033 1757936891 16 0 131231 0;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{mso-style-parent:"";
margin:0cm;
margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:12.0pt;
font-family:Arial;
mso-fareast-font-family:"MS Mincho";
mso-bidi-font-family:Arial;
mso-ansi-language:EN-GB;
mso-bidi-language:AR-SA;}
@page Section1
{size:595.3pt 841.9pt;
margin:70.85pt 3.0cm 70.85pt 3.0cm;
mso-header-margin:35.4pt;
mso-footer-margin:35.4pt;
mso-paper-source:0;}
div.Section1
{page:Section1;}
-->
Central neuropathic pain is refractory to
conventional treatment and thus remains a therapeutic challenge. In this work,
we used a well-recognized model of central neuropathic pain to evaluate
time-dependent expression of preprodynorphin (ppD), protein kinase C gamma
(PKCgamma) and NMDA receptor (NMDAR) subunits NR1, NR2A and NR2B, all critical
players in nociceptive processing at the spinal level. Male Sprague-Dawley rats
were subjected to spinal hemisection at T13 level and sham-operated rats were
included as control animals. The development of hindpaw mechanical allodynia
was assessed using the von Frey filaments test. Real time RT-PCR was employed
to determine the relative mRNA levels of NMDAR subunits, ppD and PKCgamma in
the dorsal spinal cord 1, 14 and 28 days after injury. Our results show that,
coincident with the allodynic phase after injury, there was a strong
up-regulation of the mRNAs coding for ppD, PKCgamma and NMDAR subunits in the
dorsal spinal cord caudal to the injury site. The present study provides
further evidence that these molecules are involved in the
development/maintenance of central neuropathic pain and thus could be the
target of therapeutic approaches.