INVESTIGADORES
DE NICOLA Alejandro Federico
artículos
Título:
Effects of progesterone on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin `proteins following spinal cord injury
Autor/es:
LABOMBARDA, F., GONZALEZ, S., LIMA,A. ROIG,P., GUENNOUN,R., SCHUMACHER, M. DE NICOLA, A:F:
Revista:
GLIA
Editorial:
Wiley
Referencias:
Lugar: Hoboken, NJ; Año: 2009 vol. 57 p. 884 - 888
ISSN:
0894-1491
Resumen:
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Progesterone is emerging as a myelinizing
factor for central nervous system injury. Successful remyelination requires
proliferation and differentiation of oligodendrocyte precursor cells (OPC) into
myelinating oligodendrocytes, but this process is incomplete following injury. To
study progesterone actions on remyelination, we administered progesterone (16 mg/kg/day)
to rats with complete spinal cord injury. Rats were euthanized 3 or 21 days
after steroid treatment. Short progesterone treatment: a) increased the number
of OPC without effect on the injury-induced reduction of mature
oligodendrocytes; b) increased mRNA and protein expression for the myelin basic
protein (MBP) without effects on proteolipid protein (PLP) or myelin
oligodendrocyte glycoprotein (MOG) and c) increased the mRNA for Olig2 and Nkx2.2
transcription factors involved in specification and differentiation of the
oligodendrocyte lineage. Furthermore, long progesterone treatment: a) reduced
OPC with a concomitant increase of oligodendroytesL; b) promoted
differentiation of cells that incorporated bromodeoxyuridine, early after
injury, into mature oligodendrocytes; c) increased mRNA and protein expression
of PLP without effects on MBP or MOG and d) increased mRNA for the Olig1 transcription
factor involved in myelin repair. These results suggest that early progesterone
treatment enhanced the density of OPC and induced their differentiation into
mature oligodendrocytes by increasing the expression of Olig2 and Nkx2.2. Twenty
one days after injury, progesterone favors remyelination by increasing Olig1 (involved
in repair of demyelinated lesions), PLP expression and enhancing
oligodendrocytes maturation. Thus, progesterone effects on oligodendrogenesis
and myelin proteins may constitute fundamental steps for repairing traumatic
injury inflicted to the spinal cord.
