Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation

AYALA PEÑA, VICTORIA; SANTILLÁN, GRACIELA; SCOLARO, LUIS

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2014 vol. 451 p. 609 - 614

0006-291X

In this work we characterized the infection of a primary culture of rat osteoblastic lineage 27 cells (OBCs) with measles virus (MeV) and the effect of infection on cell differentiation and 28 maturation. Infection of OBCs with MeV led to high titers of infectivity released early after 29 infection. Also, analysis of mRNAs corresponding to osteogenic differentiation markers like 30 alkaline phosphatase (ALP), bone sialo-protein (BSP) and bone morphogenetic proteins 31 (BMPs) 1-4-5-7 in OBCs revealed higher values (2 to 75-fold of increment) for infected 32 cells in comparison with uninfected controls. Differentiation of OBCs in osteogenic medium 33 prior to infection influenced the level of stimulation induced by MeV. Furthermore, 34 treatment of OBCs with Ly294002, a PI3K/AKT inhibitor, increased viral titers, whereas 35 treatment with 10 μM or 100 μM ATPS diminished MeV multiplication. In addition, 36 increments of osteogenic differentiation markers induced by MeV infection were not 37 modified either by treatment with Ly294002 or ATPS. These data provide the first 38 evidence demonstrating that MeV can infect osteoblasts in vitro leading to osteoblastic 39 differentiation, a key feature in bone pathogenic processes like otosclerosis.