INVESTIGADORES
GOMEZ CASATI Diego Fabian
artículos
Título:
Development of Fast and Simple Chromogenic Methods for Glucan Phosphatases In-Gel Activity Assays.
Autor/es:
CARRILLO J.B.; DIEGO FABIAN GOMEZ CASATI; BUSI, M. V.; MARTIN, M.
Revista:
ANALYTICAL BIOCHEMISTRY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2017 vol. 517 p. 36 - 39
ISSN:
0003-2697
Resumen:
Glucan phosphatases are essential for normal starchdegradation in plants and glycogen metabolism in mammals. Here we develop twochromogenic methods for the detection of glucan phosphatase activity in situafter non denaturing poliacrylamide gel electrophoresis; one method uses pNPPand the second one applies BCIP/NBT. The assays are sensitive, fast, simple,reliable and cost-effective preventing the use of radioactive or fluorogeniccompounds. Taking advantage of an efficient separationmethod combined with the reported assays it is possible to obtain informationabout oligomeric state of the active enzymes as well as to simultaneouslydetect glucan substrate binding and phosphatase activity
