Genome and rye chromosome distribution in tricepiro “Don René INTA”, a synthetic forage

MAIA FRADKIN, MARÍA ROSA FERRARI, MARÍA ISABEL REMIS, EDUARDO JOSÉ GREIZERSTEIN AND LIDIA POGGIO

GENETIC RESOURCES AND CROP EVOLUTION

SPRINGER

Lugar: Berlin; Año: 2012 vol. 59 p. 1281 - 1286

0925-9864

The tricepiro “Don René INTA” is an artificial hybrid with 2n = 42 chromosomes, including 14 rye (RR) and 28 wheat (AABB) chromosomes, with introgression of Thinopyrum ponticum (Podp.) Barkw. et Dewey on chromosome 6A. The aim of this work was to study the spatial distribution of the genomes and chromosomes of rye and wheat in metaphase cells from the root tip of this hybrid. The rye chromosomes were recognized by genome in situ hybridization using total genomic DNA as a probe of rye and wheat DNA as the blocking agent. Two points were determined in each cell: one representing the genomic mean distance of rye chromosomes (GRMD) and the other the genomic mean distance of wheat chromosomes (GWMD). The distance between the rye chromosomes and GRMD and GWMD showed no statistically significant difference, thus indicating that there would be no differential spatial domains for both genomes. The fluorescence in situ hybridization analysis of rye chromosomes using the pSc119.2 probe suggested that all chromosome pairs present somatic association in mitotic metaphase.