Genomic relationships among diploid and polyploid species of the genus Eryngium L. Using genomic in situ hybridization.

GISÈLE YVONNE PERTHUY, SUSANA MARTÍNEZ, EDUARDO JOSÉ GREIZERSTEIN, AND LIDIA POGGIO

GENOME

NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS

Lugar: Canada; Año: 2010 vol. 53 p. 824 - 831

0831-2796

Eryngium L. (Umbelliferae) is a large genus including more than 250 species wordwide. The large morphological variability in this genus makes it difficult to delimit the species or to establish phylogenetic relationships. The occurrence of different ploidy levels within the genus might indicate a hybrid origin of the polyploid species. In the present study, the chromosome number and karyotype of E. regnelli are reported for the firt time and the ploidy level of a population of E. megapotamicum is confirmed. We compare the genomes of the diploids E. horridum and E. eburneum, the tetraploids E. megapotamicum and E. regnellii, and the hexaploids E. pandaifolium (as a representative of the whole pandanifolium complex) and E. paniculatum using genomic in situ hybridization (GISH). Although it was not possible to identify the parental species of the polyploid taxa analyzed, the GISH technique allowes us to postulate some hypotheses about their origin. Eryngium horridum and E. eburneum do not seem to be the direct progenitors of the polyploids analyzed. On the other hand, it seems that other diploid species unrelated to E. horridum and E. eburneum are involved in their origin. Our resultad are cosistent with mophological and phylogenetic studies, indicating a close relationship between the species of the series Latifolia.