From agro-waste to tool: biotechnological characterization and application of Ganoderma lucidum E47 laccase in dye decolorization

PALAZZOLO, MARTÍN A.; POSTEMSKY, PABLO D.; KURINA-SANZ, MARCELA

3 Biotech

Springer

Año: 2019 vol. 9

2190-572X

The culture of fungalspecies from agro-waste allows for the sustainable preparation of valuablebiotechnological products and contributes to establish the Circular Economyconcept. The Ganoderma lucidumspecies is well known as producer of laccases (EC 1.10.3.2), which serve as atool to oxidize chemicals. When producing G.lucidum E47 basidiomes with edible purposes out of rice crop residues, itslaccase remains as by-product. In this work, we report the biotechnologicalcharacterization and application of the laccase recovered from spent culturesof the G. lucidum E47 strain. Wedetected at least one polypeptide (ca. 59 kDa) which displays attractiveactivity and stability values when used in the range of 18 °C to 45 °C inmildly acidic environment (pH 4.8 to 5.8). These parameters can be enhanced inthe presence of organic cosolvents like butyl acetate and methyl iso-butylketone, but the opposite effect is observed with solvents of lower log P. Thebest activity-stability performance is reached when the biocatalyst is used inpH 4.8 buffer with 5 % (v/v) butyl acetate at 37 °C. The laccasewas capable of decolorizing xanthene, azo and triarylmethane dyes, exhibitingexcellent selectivity on bromocresol green and bromocresol purple. Furthermore,the biocatalyst displayed an attractive activity when assessed for thedecolorization of bromocresol green in a proof-of-concept effluentbiotreatment.