INVESTIGADORES
GARCIA Guillermo Manuel
artículos
Título:
Antifungal activity and killing kinetics of anidulafungin, caspofungin and amphotericin B against Candida auris
Autor/es:
DUDIUK, CATIANA; BERRIO, INDIRA; LEONARDELLI, FLORENCIA; MORALES-LOPEZ, SORAYA; THEILL, LAURA; MACEDO, DAIANA; YESID-RODRIGUEZ, JOSÉ; SALCEDO, SORAYA; MARIN, ADRIANA; GAMARRA, SOLEDAD; GARCIA-EFFRON, GUILLERMO
Revista:
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Editorial:
OXFORD UNIV PRESS
Referencias:
Lugar: Oxford; Año: 2019
ISSN:
0305-7453
Resumen:
Background: Candida auris is an emerging MDR pathogen. It shows reduced susceptibility to azole drugs and, insome strains, high amphotericin B MICs have been described. For these reasons, echinocandins were proposedas first-line treatment for C. auris infections. However, information on how echinocandins and amphotericin Bact against this species is lacking.20 Objectives: Our aim was to establish the killing kinetics of anidulafungin, caspofungin and amphotericin Bagainst C. auris by time?kill methodology and to determine if these antifungals behave as fungicidal or fungistaticagents against this species.Methods: The susceptibility of 50 C. auris strains was studied. Nine strains were selected (based on echinocandinMICs) to be further studied. Minimal fungicidal concentrations, in vitro dose?response and time?kill patterns25 were determined.Results: Echinocandins showed lower MIC values than amphotericin B (geometric mean of 0.12 and 0.94 mg/L,respectively). Anidulafungin and caspofungin showed no fungicidal activity at any concentration (maximum logdecreases in cfu/mL between 1.34 and 2.22). On the other hand, amphotericin B showed fungicidal activity, butat high concentrations (2.00 mg/L). In addition, the tested polyene was faster than echinocandins at killing30 50% of the initial inoculum (0.92 versus .8.00 h, respectively).Conclusions: Amphotericin B was the only agent regarded as fungicidal against C. auris. Moreover, C. aurisshould be considered tolerant to caspofungin and anidulafungin considering that their MFC:MIC ratios weremostly32 and that after 6 h of incubation the starting inoculum was not reduced in .90%.
