Epithelial Rat Embryonic Development Exhibits Differential Glycan Expression According to Organ Localization

FERRETTI VALERIA; ISLA LARRAIN M; BARBEITO CG.; ABBA MC; SEGAL EIRAS A; CROCCE MARIA V

EC VETERINARY SCIENCE

cronicon

Año: 2018

2306-7381

Valeria 1, Marina Isla Larrain1, Claudio Barbeito2, Martin Abba1, Amada Segal-Eiras1 and Maria VirginiaCroceThe development of the different systems involved glycoproteins including mucins present in most epithelia. Mucins are glycoproteinswhich 50 - 60% of their molecular masses consisted on carbohydrates. It has been largely described changes on their glycosylationmainly related to tumors with the appearance of short carbohydrate as well as Lewis group associated antigens. At present,the expression of these carbohydrate antigens during embryonic development has not been studied. Here we have performed adetailed immunohistochemical investigation of mucin associated glycans such as Tn (GalNAcα1-O-Ser/Thr, CD175), T (Thomsen-Friedenreich, Galβ1-3GalNAcα1-O-Ser/Thr, CD176), and sTn (CD175s, sialyl-Tn antigen), Lewis x and sialyl Lewis x (sLewis x) duringrat epithelia embryonic development as well as in neonates and adults. A high expression of Tn, T, and Lewis x was found in mostintestinal organs and kidney; the skin expressed T and Lewis x antigens, salivary glands showed Tn, T and these glands were the onlytissues that expressed sTn. Respiratory organs, liver, and pancreas were negative for all analyzed antigens while sLewis x was notexpressed at any sample. In most organs, Tn, T, sTn and Le x antigens showed an increased percentage of stained cells along withdevelopmental progression. Our findings would suggest a role of carbohydrate epitopes probably modifying cell-cell and cell-matrixinteractions and cytodifferentiation during embryonic development.