INVESTIGADORES
CORREA Silvia Graciela
artículos
Título:
Lipase of Candida albicans induces activation of NADPH oxidase and L-arginine pathways on resting and activated macrophages.
Autor/es:
PARAJE MG, CORREA SG, ALBESA I, SOTOMAYOR CE.
Revista:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Año: 2009 vol. 390 p. 263 - 268
ISSN:
0006-291X
Resumen:
Candida albicans secretes various hydrolytic enzymes which are considered to be an integral part in the 22albicans secretes various hydrolytic enzymes which are considered to be an integral part in the 22
pathogenesis. However, the role of lipases is far from being completely understood and the direct effects 23However, the role of lipases is far from being completely understood and the direct effects 23
of these fungal enzymes during the hostpathogen interaction remain to be established. We recently iso- 24hostpathogen interaction remain to be established. We recently iso- 24
lated and characterized an extracellular C. albicans lipase (CaLIP), and demonstrated the ability of this 25C. albicans lipase (CaLIP), and demonstrated the ability of this 25
fungal enzyme to interact directly with macrophages (M/) and hepatocytes and to operate as a virulence 26/) and hepatocytes and to operate as a virulence 26
factor. Herein, we explored the effects of CaLIP on M/ functions such as oxidative burst and L-arginine 27Herein, we explored the effects of CaLIP on M/ functions such as oxidative burst and L-arginine 27
metabolism. The study was performed in cells with different activation status: normal-resting M/s 28/s 28
and M/s primed in vivo or in vitro with C. albicans. The ability of this fungal factor to modulate the 29/s primed in vivo or in vitro with C. albicans. The ability of this fungal factor to modulate the 29
above-mentioned parameters was dependent on cells status, dose, and microenvironment, where the 30parameters was dependent on cells status, dose, and microenvironment, where the 30
interaction took place. These results constitute a new finding in the biology of candidiasis and could illus- 3131
trate an additional evolutive advantage for the fungus in the framework of the bidirectional hostpath- 32hostpath- 32
ogen interaction.interaction.