IL-1β, IL-2 and IL-4 concentration during porcine gestation

VÉLEZ, CAROLINA; CLAUZURE, MARIÁNGELES; WILLIAMSON, DELIA; KONCURAT, MIRTA A.; SANTA-COLOMA, TOMÁS A.; BARBEITO, CLAUDIO

THERIOGENOLOGY

ELSEVIER SCIENCE INC

Año: 2019 vol. 128 p. 133 - 139

0093-691X

In pigs, given the type of epitheliochorial and non-invasive placenta, the trophoblast is in intimatecontact with maternal tissues. The dialogue established between the conceptus and the endometriuminvolves, among others, the immune system, which minimizes the chances of rejection of the embryoand promotes the establishment of pregnancy. The aim of this work was to determine the concentrationof IL-1b, IL-2 and IL-4 in sera and in extracts of maternal and fetal placenta from sows of differentgestational periods. Reproductive tracts from 23 crossbreed sows, between 30 and 114 days of gestation(dg), and from 8 non-pregnant sows were used. The concentration of the cytokines was determined byELISA. IL-1b, IL-2 and IL-4 demonstrated a similar pattern of concentration at the placental interface andserum; they were found elevated in tissues at 30 and 60e70 dg, and significantly decreased at term,period in which the cytokines were significantly increased in serum. These results show that IL-1b, IL-2,and IL-4 are differentially modulated during pregnancy and at term, and suggest an important role ofthese cytokines in defining the proinflammatory stage of these periods.