Freely Available Tool (FAT) for automated quantification of lipid droplets in stained cells

MASONE, DIEGO; GOJANOVICH, ALDANA D.; FRONTINI, YESICA; DEL VELIZ, SAMANTA; UHART, MARINA; BUSTOS, DIEGO M

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2017 vol. 41 p. 55 - 58

0327-9545

Abstract: In this study, we propose an automatic procedure for digital image processing. We describe a method that canefficiently quantify and characterize lipid droplets distributions in different cell types in culture. Prospectively, the lipiddroplets detection method described in this work could be applied to static or time-lapse data, collected with a simplevisible light or fluorescence microscopy equipment. Fully automated algorithms were implemented in Octave, a freelyavailable scientific package.