From fish-processing waste to feed additives for crayfish

RODRIGUEZ YE; SACRISTAN HJ; MARÍA VICTORIA LAITANO; LOPEZ GRECO L.S; FERNANDEZ GIMENEZ AV; FERNANDEZ GIMENEZ AV

JOURNAL OF THE WORLD AQUACULTURE SOCIETY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2019 p. 1 - 15

0893-8849

This research studied thein vitrocompatibility ofCherax quadricarina-tusdigestive proteases and crude enzyme extracts recovered fromfish-processing waste to evaluate their potential use as feed additivesto increase digestion efficiency in decapod crustaceans. The sources ofexogenous enzymes were:Pleoticus muelleri(Pm), Artemesia longinaris(Al), andIllex argentinus(Ia). The midgut glands of early (7.20.34 g)and advanced juveniles (13.81.49 g) ofC. quadricarinatuswere usedfor all the assays. The compatibilityandsynergybetweencrayfishandexogenous enzymes were evaluated through sodium dodecyl sulfate?polyacrylamide gel electrophoresis (SDS?PAGE) electrophoresis andthe hydrolysis of azocasein and four different meals. SDS?PAGE gelsdemonstrated that crayfish bands remained active when their enzymeswere combined with the enzymes from Pm and Ia. An apparent enzy-matic synergism was found when Pm and crayfish proteases wereincubated together at pH 6 and 8 with azocasein (p˂0.05). The abilityto hydrolyze the different feed ingredients varied among the enzymecombinations assayed. Our findings suggest that fish waste has poten-tial as a source of proteases, adding value to these processing residuesand promoting a zero-waste industry. Enzymes obtained from Pm andIa are suitable to be used as food additives for crayfish. The resultsshould be validated through feeding trials