Active compounds present in Rosmarinus officinalis leaves and Scutellaria baicalensis root evaluated as new therapeutic agents for endometriosis

FERELLA, LUCIANA; BASTÓN, JUAN IGNACIO; BILOTAS, MARIELA ANDREA; SINGLA, JOSÉ JAVIER; GONZÁLEZ, ALEJANDRO MARTÍN; OLIVARES, CARLA NOEMÍ; MERESMAN, GABRIELA FABIANA

REPRODUCTIVE BIOMEDICINE ONLINE

REPRODUCTIVE HEALTHCARE LTD

Lugar: Amsterdam; Año: 2018

1472-6483

Research question: Can carnosic acid, (CA) rosmarinic acid (RA) and wogonin (WG) inhibit thegrowth of cultured human endometrial stromal cells and endometriotic-like lesions induced in aBALB/c model of endometriosis?Design: Primary stromal cell cultures were established from endometrial biopsies from womenwith endometriosis and controls. The human endometrial stromal cell line T-HESC was also usedfor in-vitro experiments. Endometriosis was surgically induced in BALB/c mice, which wererandomly assigned to CA 2 mg/kg/day (n = 11); CA 20 mg/kg/day (n = 10); RA 1 mg/kg/day (n= 11); RA 3 mg/kg/day (n = 10); WG 20 mg/kg/day (n = 12); intraperitoneal vehicle control (n =8) or oral vehicle control (n = 11). After surgery, CA and RA were administered intraperitoneallyon days 14?28. WG was administered orally by intragastric gavage on days 14?26.ACCEPTED MANUSCRIPTACCEPTED MANUSCRIPT2Results: CA, RA and WG significantly inhibited in-vitro cell proliferation in primary and THESCcell cultures (P < 0.05). CA and WG induced cell cycle arrest of T-HESC at the G2/Mphase (P < 0.01). RA reduced intracellular ROS accumulation (P < 0.001), whereas WGincreased it (P < 0.05). WG significantly inhibited oestrogen receptor alpha expression in THESC(P < 0.01). In-vivo, CA, RA and WG significantly reduced lesions size (P < 0.05). Allcompounds significantly decreased the percentage of cells in proliferation (P < 0.05) whereas RAand WG further increased the percentage of apoptotic cells (P < 0.05) in endometriotic-likelesions.Conclusions: The results are promising; further investigation of these compounds as newtherapeutics is needed.