Evaluation of kinetic behavior of two preparations of tylosin administered in beehives for American Foulbrood control

REYNALDI F.J.; ALBO G.N.; AVELLANEDA E.; RULE R.

Bulgarian Journal of Veterinary Medicine

An Official Publication of the Faculty of Veterinary Medicine, Trakia University Stara Zagora, Bulgaria

Lugar: Sofia; Año: 2017 vol. 20 p. 264 - 270

We compared the kinetic behavior of tylosin (TY) administered to beehives by dusting or paper-pack placement through three treatment protocols (T1, T2, T3). T1 (Dusting): TY, divided in four portions, was sprinkled over the ends of the hives´ top bars weekly for four weeks (n=3); T2 (Paper-pack placement): TY in paper packs was administered at two-week intervals (n=3); T3 (Control): The hives were left untreated (n=3). In every inspection, from each of the nine hives, fifty young (2-day-old) larvae were sampled for drug analysis. The concentration of TY in the young larvae was determined by a microbiological assay with Geobacillus stearothermophilus ATCC 12.980 as the test organism. Results (Mean ± SD): the maximum concentration (Cmax) for T1 was 136.0 ± 194.0 for T2 144.0 ± 187.4 μg/ml; the time to reach the Cmax (tmax) for T1 was 1.5 ± 0.9, for T2 1.8 ± 1.8 h; the area under the TY-behavior-kinetics curve between 0-1392 h with T1 was 308.7 ± 185.2, with T2 326.4 ± 141.0 μg/h/ml], indicating no statistical difference between the treatments (p>0.05). The shorter duration of paper-pack-administered TY observed in the larvae implied a lower risk of antibiotic residues in the resulting honey.