INVESTIGADORES
GORLA Nora Bibiana Maria
artículos
Título:
GENOTOXICITY OF AMPA, ENVIRONMENTAL METABOLITE OF GLYPHOSATE, ASSESSED BY THE COMET ASSAY AND CYTOGENETIC TESTS
Autor/es:
MAÑAS, F. PERALTA, L. RAVIOLO, J.GARCÍA OVANDO, H., WEYERS, A., UGNIA, L., GONZALEZ CID M., LARRIPA I., GORLA, N.,
Revista:
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Año: 2009 vol. 72 p. 834 - 837
ISSN:
0147-6513
Resumen:
a b s t r a c t
Formulations containing glyphosate are the most widely used herbicides in the world. AMPA is the
major environmental breakdown product of glyphosate. The purpose of this study is to evaluate the in
vitro genotoxicity of AMPA using the Comet assay in Hep-2 cells after 4 h of incubation and the
chromosome aberration (CA) test in human lymphocytes after 48 h of exposition. Potential in vivo
genotoxicity was evaluated through the micronucleus test in mice. In the Comet assay, the level of DNA
damage in exposed cells at 2.57.5mM showed a significant increase compared with the control group.
In human lymphocytes we found statistically significant clastogenic effect AMPA at 1.8mM compared
with the control group. In vivo, the micronucleus test rendered significant statistical increases at
200400mg/kg. AMPA was genotoxic in the three performed tests. Very scarce data are available about
AMPA potential genotoxicity.