Biomass-bound biocatalysts for biodiesel production: Tuning a lipolytic activity from Aspergillus niger MYA 135 by submerged fermentation using agro-industrial raw materials and waste products

ERIKA LUCÍA REGNER, HEBE NATALIA SALVATIERRA, MARIO BAIGORÍ AND LICIA PERA

BIOMASS AND BIOENERGY

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2019 vol. 120 p. 59 - 67

0961-9534

The enzymatic route is a promising strategy to synthesize biodiesel essentially because of its moderate reactionconditions and easy product separation. Additionally, coupling the biodegradation of agro-industrial raw materialsand waste products with the enzyme production has also had a significant impact on the development ofindustrial biotechnology. This is mainly due to the low-cost and the prevention of pollution they represent. Inthis work, different kinds of residues and wastes were used for tuning biomass-bound lipolytic biocatalysts fromAspergillus niger MYA 135 by submerged fermentation using the Plackett-Burman design. As a result, by a specificcombination of medium components, two distinctive biocatalysts were obtained. Thus, the production of amycelium-bound esterase was maximized in the presence of (in g l−1): CaCl2 0.5, FeCl3 1.0, corn meal 2.0,cerelose 10.0, peptone 10.0, and waste cooking oil 10.0. While, the production of a mycelium-bound lipase wasselectively increased in the presence of (in g l−1): molasses 5.0, vinasse 5.0, CaCl2 0.5, FeCl3 1.0, milk serum10.0, cerelose 5.0, peptone 5.0 and waste cooking oil 10.0. Besides, the biomass-bound lipase activity producedin the optimized culture medium was also prepared either free or immobilized in alginate beads, and thenindividually placed in small-scaled packed-bed reactors to produce biodiesel compounds in a solvent-free systemusing soybean oil and butanol as substrates. After a three-stepwise addition of butanol, a biodiesel conversion of57.32 and 95.89% was reached in the presence of free and immobilized biomass, respectively. Biocatalystsmorphologies were also analyzed.