Computational prediction of nsSNPs effects on bovine GSTP1: an integrated protein function and structure approach to SNPs prioritization

FALOMIR-LOCKHART, A.H.; VILLEGAS-CASTAGNASO, E.E.; GIOVAMBATTISTA, G.; ROGBERG-MUÑOZ, A.

FREE RADICAL BIOLOGY AND MEDICINE

ELSEVIER SCIENCE INC

Año: 2018 p. 486 - 491

0891-5849

The glutathione S-transferases (GSTs) superfamily protects against reactive species and substances, minimizing oxidative stress. Additionally, it regulates the apoptotic processes through a non-enzymatic interaction with signaling kinases. Previous studies have shown that GSTP1 haplotypes produce differences on cellular proliferation and apoptosis. Therefore, analyzis of the genetic variability and of the potential impact of amino acid variants on protein structure and function by cost-effective and reliable in silico methods could enhance our understanding of GSTP1 involvement in muscle redox state and apoptosis. In particular, post mortem conversion of animal muscle into meat involves apoptosis and could be used as a model of cellular extreme oxidative stress leading to programmed cell death. In this research, bovine GSTP1 gene was studied, fifteen exonic SNPs were detected and their effect was evaluated using seven programs. Our results were consistent, showing that substitutions C15W, C48G, Q65H and L70Q may impact on GSTP1 structure and function. Moreover, haplotype analyzis enabled the prioritization of three structures for further analyzis. This is one of the first attempts to study residue variants in bovine GSTP1 protein using prioritization bioinformatics tools before conducting in vitro experimental studies.