Análisis retrospectivo de casos con diagnóstico de necrosis cerebrocortical y su relación con herpesvirus bovino tipo 5.

PÉREZ, S; VAGNOZZI, A; SUR, J; ODRIOZOLA, E; CAMPERO, C; ODEÓN, A

REVISTA ARGENTINA DE MICROBIOLOGíA

Asociación Argentina de Microbiología

Año: 2003 vol. 35 p. 69 - 93

0325-7541

In order to demonstrate the association between bovine herpesvirus type 5 (BHV-5) and cerebrocortical necrosis (CCN), 89 such cases were examined in cattle from 3 regions of Buenos Aires Province, Argentina, during 1970-99. Haematoxylin-eosin staining and BHV-5 in situ hybridization were performed on paraffin-embedded neural tissues. The severity of microscopic lesions was scored according to a 0-3 scale. Morbidity, mortality and lethality rates between groups depending on age and regions were determined. The highest prevalence of CCN was detected between 1979 and 1984, particularly during the spring. Differences in morbidity and mortality rates between groups of age and regions were not detected (P>0.05). Amaurosis (48%), ptyalism (42%), circling (40%), ataxia (36%) and bruxism (37%) were frequently observed. Lesions were predominantly found in anterior and posterior cortex (90.6%) and diencephalon (36.5%). Meningitis and perivascular cuffing (94.4%) and focal (78%) or diffuse(73%) gliosis were predominant in the cerebrum. Focal necrosis was observed in 66.6% of cases. BHV-5 was isolated in 9 of 19 cases since 1992 and BHV-5 DNA was detected by in situ hybridization in 3 of 9 cases. No virus was identified in brain tissues with severe lesions. These findings indicate the association of BHV-5 in neurological disease previously reported as CCN