INVESTIGADORES
PEREZ Sandra Elizabeth
artículos
Título:
Dexamethasone treatment of calves latently infected with bovine herpesvirus 1 leads to the activation of the bICP0 early promoter, in part by the cellular transcription factor (C/EBP-a).
Autor/es:
WORKMAN, ASPEN; PÉREZ, S; DOSTER, A; JONES, C
Revista:
JOURNAL OF VIROLOGY
Editorial:
American Society for Microbiology
Referencias:
Año: 2009 vol. 83 p. 8800 - 8809
ISSN:
0022-538X
Resumen:
Sensory neurons within trigeminal ganglia (TG) are the primary site for bovine herpesvirus 1 (BHV-1)
latency. During latency, viral gene expression is restricted to the latency-related (LR) gene and the open
reading frame ORF-E. We previously constructed an LR mutant virus that expresses LR RNA but not any of
the known LR proteins. In contrast to calves latently infected with wild-type (wt) BHV-1 or the LR rescued
virus, the LR mutant virus does not reactivate from latency following dexamethasone (DEX) treatment. In this
study, we demonstrated that bICP0, but not bICP4, transcripts were consistently detected in TG of calves
infected with the LR mutant or LR rescued virus following DEX treatment. Calves latently infected with the LR
rescued virus but not the LR mutant virus expressed late transcripts, which correlated with shedding of
infectious virus following DEX treatment. The bICP4 and bICP0 genes share a common immediate-early
promoter, suggesting that this promoter was not consistently activated during DEX-induced reactivation from
latency. The bICP0 gene also contains a novel early promoter that was activated by DEX in mouse neuroblastoma
cells. Expression of a cellular transcription factor, C/EBP-alpha, was stimulated by DEX, and C/EBPalpha
expression was necessary for DEX induction of bICP0 early promoter activity. C/EBP-alpha directly
interacted with bICP0 early promoter sequences that were necessary for trans activation by C/EBP-alpha. In
summary, DEX treatment of latently infected calves induced cellular factors that stimulated bICP0 early
promoter activity. Activation of bICP0 early promoter activity does not necessarily lead to late gene expression
and virus shedding.
summary, DEX treatment of latently infected calves induced cellular factors that stimulated bICP0 early
promoter activity. Activation of bICP0 early promoter activity does not necessarily lead to late gene expression
and virus shedding.
trans activation by C/EBP-alpha. In
summary, DEX treatment of latently infected calves induced cellular factors that stimulated bICP0 early
promoter activity. Activation of bICP0 early promoter activity does not necessarily lead to late gene expression
and virus shedding.