INVESTIGADORES
BEKINSCHTEIN Pedro Alejandro
artículos
Título:
One-trial aversive learning induces late changes in hippocampal CaMKIIalpha, Homer 1a, Syntaxin 1a and ERK2 protein levels.
Autor/es:
LIONEL MÜLLER IGAZ; PEDRO BEKINSCHTEIN; IVAN IZQUIERDO; JORGE H. MEDINA
Revista:
MOLECULAR BRAIN RESEARCH
Referencias:
Año: 2004 vol. 132 p. 1 - 12
ISSN:
0169-328X
Resumen:
Most studies regarding altered gene expression after learning are
performed using multi-trial tasks, which do not allow a clear
discrimination of memory acquisition, consolidation and retrieval. We
screened for candidate memory-modulated genes in the hippocampus at 3
and 24 h after one-trial inhibitory avoidance (IA) training, using a
cDNA array containing 1176 genes. While 33 genes were modulated by
training (respect to shocked-only animals), most of them were
upregulated (27 genes) and only 6 were downregulated. To confirm and
extend these findings, we performed RT-PCRs and analyzed differences in
protein levels in rat hippocampus using immunoblot assays. We found
several proteins upregulated 24 h after training: extracellular
signal-regulated kinase ERK2, Ca2+/calmodulin-dependent protein kinase
II alpha (CaMKIIalpha), Syntaxin 1a, c-fos and Homer 1a. The total
level of none of these proteins were found to be altered when measured
3-h post-training. Several of the mRNAs corresponding to the
upregulated proteins were changed at 3 h but not 24 h. Additionally, a
number of other candidates were identified for the first time as
modulated by learning. The results presented here suggest that
single-trial tasks can expose previously unseen differences in dynamic
regulation of gene expression after behavioral manipulations, both at
the transcriptional and translational levels, and reveal a diversity of
gene products modulated by this task, allowing deeper understanding of
the molecular basis of memory formation.