Metagenomic approach of associated fungi with Megaplatypus mutatus (Coleoptera: Platypodinae)

ESTEBAN CERIANI-NAKAMURAKARE; SERGIO RAMOS; CAROLINA A. ROBLES; MARÍA V. NOVAS; MARÍA F. D´JONSILES; PAOLA GONZALEZ-AUDINO; CECILIA CARMARÁN

SILVA FENNICA

FINNISH SOC FOREST SCIENCEFINNISH FOREST RESEARCH

Año: 2018 vol. 52 p. 1 - 16

0037-5330

Megaplatypus mutatus is a major forest pest in Argentina and an emerging pest in Europe. In thisstudy the multitrophic interactions between M. mutatus and associated fungi were assessed witha metagenomics approach (454-pyrosequencing). A total of 270 collection points from insectgalleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts,Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterizethe fungal communities associated to M. mutatus; compare the culture-independent approach withprevious culturing studies, in terms of data recovery related to the fungal community composition,and test the specificity of the fungal communities amongst locations and hosts. A GeneralizedLinear Mixed Model was performed to compare the fungal richness in each dataset, whichshowed no significant differences between taxa richness amongst locations. Principal CoordinatesAnalyses showed a separation between fungal communities within the same host, suggesting thathost identity would not be crucial to determine the specificity in fungal communities. Candidainsectalens and one Fusarium species, present in all hosts and locations, achieved 37.6% of thetotal relative frequency per taxa. These results complement the data from culturing methodspreviously reported, thus improving the accuracy and understanding of the fungal assemblagesassociated to M. mutatus