IDIM   12530
INSTITUTO DE INVESTIGACIONES MEDICAS
Unidad Ejecutora - UE
artículos
Título:
pATTERNS OF RENAL DOPAMINE RELEASE TO REGULATE DIURESIS AND NATRIURESIS DURING VOLUME EXPANSION.ROLE OF RENAL MONOAMINE-OXIDASE
Autor/es:
VERÓNICA DE LUCA SAROBE; LUIS DI CIANO; ANDREA M CARRANZA; GLORIA LEVIN; ELVIRA E ARRIZURIETA; FERNANDO R IBARRA
Revista:
MEDICINA (BUENOS AIRES)
Editorial:
MEDICINA (BUENOS AIRES)
Referencias:
Lugar: Buenos Aires; Año: 2010 vol. 70 p. 60 - 64
ISSN:
0025-7680
Resumen:
Abstract Diuretic and natriuretic effects of renal dopamine (DA) are well established. However, in volume
expansion the pattern of renal DA release into urine (UDAV) and the role of enzymes involved in DA
synthesis/degradation have not yet been defined. The objective is to determine the pattern of UDAV during volume
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
synthesis/degradation have not yet been defined. The objective is to determine the pattern of UDAV during volume
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
expansion the pattern of renal DA release into urine (UDAV) and the role of enzymes involved in DA
synthesis/degradation have not yet been defined. The objective is to determine the pattern of UDAV during volume
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
synthesis/degradation have not yet been defined. The objective is to determine the pattern of UDAV during volume
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
Diuretic and natriuretic effects of renal dopamine (DA) are well established. However, in volume
expansion the pattern of renal DA release into urine (UDAV) and the role of enzymes involved in DA
synthesis/degradation have not yet been defined. The objective is to determine the pattern of UDAV during volume
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
synthesis/degradation have not yet been defined. The objective is to determine the pattern of UDAV during volume
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
DAV) and the role of enzymes involved in DA
synthesis/degradation have not yet been defined. The objective is to determine the pattern of UDAV during volume
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
peak to 3.2±0.72 (p < 0.01) and though, UDAV increased over C after 60 min expansion, natriuresis and diuresis were
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
diminished by BNZ treatment. Results indicate that an increment in renal DA release into urine occurs early in expansion
and in a peak-shaped way. In this response MAO plays a predominant role.
DAV during volume
expansion and to characterize the involvement of monoamine-oxidase (MAO) and aromatic amino-acid
decarboxylase (AADC) in this response. In this study male Wistar rats were expanded with NaCl 0.9% at a rate
of 5% BWt per hour. At the beginning of expansion three groups received a single drug injection as follows: C (vehicle,
Control), IMAO (MAO inhibitor Pargyline, 20 mg/kg BWt, i.v.) and BNZ (AADC inhibitor Benserazide, 25 mg/kg
BWt, i.v.). Results revealed that in C rats UDAV (ng/30 min/100g BWt) increased in the first 30 min expansion from
11.5 ± 1.20 to 21.8 ± 3.10 (p < 0.05) and decreased thereafter. IMAO showed a similar pattern but significantly higher
than C at 30 min expansion (32.5 ± 2.20, p < 0.05). IMAO greatly reduced MAO activity from 8.29 ± 0.35 to 1.1 ± 0.03
nmol/mg tissue/hour and significantly increased diuresis and natriuresis over controls. BNZ abolished the early UDAV
peak to 3.2±0.72 (p < 0.01) and though, U