INVESTIGADORES
ROZENFELD paula Adriana
artículos
Título:
Evaluation of the residual antigenicity and allergenicity of cow's milk substitutes by in vitro tests
Autor/es:
DOCENA GH; ROZENFELD PA; FERNANDEZ R; FOSSATI CA
Revista:
ALLERGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Montpellier; Año: 2002 vol. 57 p. 83 - 91
ISSN:
0105-4538
Resumen:
Background: This study aimed the to investigate presence of residual allergenic
cows milk proteins (CMP) in some milk substitutes employed in the treatment of
cows milk allergy (CMA). These allergens may interfere with the treatment, and
elicit allergic reactions in sensitized individuals.This study aimed the to investigate presence of residual allergenic
cows milk proteins (CMP) in some milk substitutes employed in the treatment of
cows milk allergy (CMA). These allergens may interfere with the treatment, and
elicit allergic reactions in sensitized individuals.
Methods: The protein composition of the different extracts was evaluated by
Lowrys method and tricine SDS-PAGE. Different immunoenzymatic methods
were used (ELISA, EAST and immunoblotting) to quantify total serum IgE and
specific serum IgE, as well as to detect the presence of antigenic and allergenic
components.The protein composition of the different extracts was evaluated by
Lowrys method and tricine SDS-PAGE. Different immunoenzymatic methods
were used (ELISA, EAST and immunoblotting) to quantify total serum IgE and
specific serum IgE, as well as to detect the presence of antigenic and allergenic
components.
Results: The results showed a higher protein content in mammalian milks (cow,
sheep, mare, goat, and human) than in hydrolyzed substitutes (partially or
extensively hydrolyzed casein or whey proteins). Residual native, processed, or
contaminant polypeptides have been identified in the moderate hydrolysates,
whereas extensive hydrolysates did not show the presence of residual
components by immunoblotting. However, specific antibodies with capacity to
bind to peptides have been detected by EAST and ELISA, suggesting that
extensive hydrolysates contain residual peptides that preserve immunoreactive
epitopes. We were unable to demonstrate either residual antigenicity or
allergenicity in an amino-acid-based formula.The results showed a higher protein content in mammalian milks (cow,
sheep, mare, goat, and human) than in hydrolyzed substitutes (partially or
extensively hydrolyzed casein or whey proteins). Residual native, processed, or
contaminant polypeptides have been identified in the moderate hydrolysates,
whereas extensive hydrolysates did not show the presence of residual
components by immunoblotting. However, specific antibodies with capacity to
bind to peptides have been detected by EAST and ELISA, suggesting that
extensive hydrolysates contain residual peptides that preserve immunoreactive
epitopes. We were unable to demonstrate either residual antigenicity or
allergenicity in an amino-acid-based formula.
Conclusions: Immunoenzymatic methods were used to detect the presence of
cross-reactive components in mammalian milks. Residual allergenic components
from cows milk could be identified in both the moderate and extensive
hydrolysates analyzed. This information may be relevant to the treatment of
CMA.Immunoenzymatic methods were used to detect the presence of
cross-reactive components in mammalian milks. Residual allergenic components
from cows milk could be identified in both the moderate and extensive
hydrolysates analyzed. This information may be relevant to the treatment of
CMA.