Direct quantification of monohydroxy-polycyclic aromatic hydrocarbons in urine samples via Solid-Phase Extraction Room-Temperature Fluorescence Excitation-Emission Matrix spectroscopy

VATSAVAI, K; GOICOECHEA, HÉCTOR C; ANDRES CAMPIGLIA,

ANALYTICAL BIOCHEMISTRY

Elsevier

Lugar: Amsterdam; Año: 2008 p. 213 - 220

0003-2697

A screening method is presented for six biomarkers from polycyclic aromatic hydrocarbonsexposure in urine samples. Solid-phase extraction is carried out on commercial C18 cartridges via anoptimized procedure that minimizes metabolite loss. PAH metabolites are directly determined in the elutingsolvent (3mL of methanol) without the need of previous solvent evaporation. Spectral overlapping isresolved with the combination of unfolded partial least-squares and residual bi-linearization. Excellentanalytical figures of merit were obtained for all the studied metabolites. Analytical recoveries varied between87.9% (9-hydroxyphenanthrene) and 99.4% (3-hydroxybenzo[a]pyrene). For 10mL of urine sample, thelimits of detection varied between 0.01ng.mL-1 (3-hydroxybenzo[a]pyerene and 1-hydroxybenzopyrene) and0.3ng.mL-1 (2-hydroxynaphthalene). Because the chemometric algorithm is capable to handle more than sixmetabolites at once, the application of this approach to a larger number of metabolites is feasible