INVESTIGADORES
PIURI Mariana
artículos
Título:
FLUOROMYCOBACTERIOPHAGES CAN DETECT VIABLE Mycobacterium tuberculosis AND DETERMINE PHENOTYPIC RIFAMPICIN RESISTANCE IN 3-5 DAYS FROM SPUTUM COLLECTION
Autor/es:
LILIANA RONDON; ESTEFANIA URDANIZ; LATINI CECILIA; PAYASLIAN FLORENCIA; MARIO MATTEO; EZEQUIEL SOSA; DARÍO FERNANDEZ DOPORTO; TURJANSKI A; SERGIO NEMIROVSKY; GRAHAM F HATFULL; SUSANA POGGI; MARIANA PIURI *
Revista:
Frontiers in Microbiology
Editorial:
Lausanne : Frontiers Research Foundation
Referencias:
Año: 2018
ISSN:
1664-302X
Resumen:
Fluoromycobacteriophage methodology is compatible with regularly used protocols in clinical laboratories for TB diagnosis and paraformaldehyde fixation after infection reduces biohazard risks with sample analysis by fluorescence microscopy. We have also set up conditions for discrimination between M. tuberculosis complex (MTBC) and non tuberculous mycobacteria (NTM) strains by addition of p-nitrobenzoic acid (PNB) during the assay.Using clinical isolates of pre-XDR and XDR-TB strains from this study, we tested mCherrybombɸ for extended DST and we compared the antibiotic resistance profile with those predicted by whole genome sequencing. Our results emphasize the utility of a phenotypic test for M. tuberculosis extended DST. The many attributes of mCherrybombɸ suggests this could be a useful component of clinical microbiological laboratories for TB diagnosis and since only viable cells are detected this could be a useful tool for monitoring patient response to treatment.