The insecticides chlorpyrifos and acetamiprid induce redox imbalance in umbilical cord blood erythrocytes in vitro

GUIÑAZÚ, NATALIA; RIVERO OSIMANI, VALERIA; ROVEDATTI, MARÍA GABRIELA; QUINTANA, MARIA MARTHA; MAGNARELLI, GLADIS

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2018 vol. 148 p. 87 - 92

0048-3575

Organophosphate and neonicotinoid compounds belong to different pesticide families, and are used worldwidefor agricultural purposes. The potential deleterious health effects associated with pesticide exposure duringpregnancy have become a major public health concern. The present study analyzed the effects of the organophosphatechlorpyrifos and the neonicotinoid acetamiprid on oxidative stress biomarkers in human umbilicalcord blood erythrocytes (UCBE). The reactive oxygen species (ROS) levels, the catalase (CAT) and superoxidedismutase (SOD) activities, the levels of 4-hidroxynonenal (HNE) and UCBE osmotic fragility were determined.Both pesticides modified the oxidative status of UCBE. Chlorpyrifos increased ROS levels at 40 and 400 nM,while only decreased CAT activity at the higher concentration assayed (400 nM), with no modification in SODactivity. The insecticide acetamiprid increased ROS levels at all concentrations assayed, and decreased CAT andSOD activity at 40 and 400 nM, Chlorpyrifos and acetamiprid (40 and 400 nM) modified HNE content. Nonsignificantchanges in UCBE osmotic fragility were induced by chlorpyrifos or acetamiprid treatments.In conclusion, both pesticides assayed increased ROS production and decreased anti-oxidant enzyme activityin UCBE, even though changes were of different extent and depended on the insecticide analyzed. Interestinglyno changes in erythrocyte osmotic fragility were registered; suggesting that the oxidative stress triggered underthese experimental conditions was not sufficient to induce a functional damage to the UCBE membrane.