INVESTIGADORES
DARDANELLI Marta Susana
artículos
Título:
Synthesis, acumulation and hydrolysis of trehalose during growth of peanut rhizobia in hyperosmotic media
Autor/es:
DARDANELLI M.S.; GONZÁLEZ P.S.; BUENO M.A.; GHITTONI N.E.
Revista:
Journal of Basic Microbiology
Editorial:
Wiley-VCH Verlag GmbH & Co. KGaA
Referencias:
Año: 2000 vol. 40 p. 149 - 156
ISSN:
1521-4028
Resumen:
We examined and compared the activities of synthetic and hydrolytic enzymes involved in trehalose
metabolism, in three peanut rhizobia strains grown in control, hypersaline, and non-ionic hyperosmotic
media. Results indicated that the effects of hyperosmolarity on the synthesis and the degradation
of the disaccharide were diverse. In the salt-tolerant slow-growing strain Bradyrhizobium sp.
ATCC 10317, we observed increased synthesis and accumulation of trehalose under hyperosmolarity
imposed by either NaCl or PEG-8000. In the other two peanut rhizobia strains, the disaccharide level
did not change under hypersalinity. In the salt-sensitive slow-growing strain Bradyrhizobium sp.
USDA 3187, intracellular trehalose diminished in late stationary phase-cells grown with PEG, this
reduction was accompanied by both an increased activity of synthetic enzymes and a decreased activity
of trehalase. In the salt-tolerant fast-growing strain Rhizobium sp. TAL 1000, we also observed a
reduction of intracellular trehalose under PEG-mediated growth, this decrease was early and transiently
accompanied by an enhancement of trehalase activity, afterwards, the activity of synthetic
enzymes augmented.