INVESTIGADORES
CORBALAN Natalia Soledad
artículos
Título:
The tolC locus affects the expression of sbmA through sE activity increase
Autor/es:
CORBALÁN, NATALIA S.; ADLER, CONRADO; DE CRISTÓBAL, RICARDO E.; POMARES, MARÍA FERNANDA; DELGADO, MÓNICA A.; VINCENT, PAULA A.
Revista:
FEMS MICROBIOLOGY LETTERS
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 p. 185 - 192
ISSN:
0378-1097
Resumen:
ABSTRACT SbmA protein is involved in the transport of MccB17, MccJ25, bleomycin and proline rich peptides into the Escherichia coli cytoplasm. sbmA gene homologues were found in a variety of bacteria. However, the physiological role of this protein is, until now, unknown. Previously, we found that a combination of sbmA and tolC mutations in Tn10?carrying E. coli K-12 strains results in hypersusceptibility to tetracycline. In this work, we studied sbmA expression in a tolC mutant background and observed an increased expression throughout growth. We ruled out the global transcriptional regulator RpoS and the sRNA micF as intermediates in this regulation. tolC mutation induced the expression of other well characterized strong sE-dependent promoters in E. coli. We observed that the increase in sE activity led to greater sbmA expression and, conversely, eliminating sE prevented expression of sbmA. We also observed that the sbmA upregulation in a tolC mutant context was abolished in an rpoE-null strain. These results suggest a sE-dependent positive regulation on sbmA by tolC mutation. We hypothesize that this mechanism might be part of a compensatory cell envelope stress response.