Application of Synthetic Tumor-Specific Promoters Responsive to the Tumor Microenvironment

CAFFERATA, E.G.A; LOPEZ MV; FELIPE J NUÑEZ; AGOSTINA RUSSO MAENZA; OSVALDO L PODHAJCER

METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)

clifton

Año: 2017

1064-3745

Activity of endogenous promoters can be altered by including additional responsive elements (REs). These elements can be responsive to features of the tumor environment or alternatively to signaling pathways specifically activated in cancer cells. These REs incorporated into tumor-specific promoters can improve cancer targeting, the replicative capacity, and lytic activity of conditionally replicative adenovirus. Here we outline an approach to incorporate hypoxia and inflammation REs into a specific fragment of the SPARC promoter and the steps to clone a nucleosome positioning sequence (NPS ) identified in the osteocalcin promoter that contains a Wnt RE upstream of a heterologous synthetic promoter.